Total cellular RNA was isolated with Purelink RNA mini kit (Fisher Scientific; Cat. No.12183018A) according to manufacturer’s instructions. RNA was used to synthesized cDNA with the Superscript IV Vilo master mix with ezDNase enzyme (Fisher Scientific; Cat. No. 11766050). Quantitative SYBR green PCR assay was performed using Powerup SYBR green master mix (Fisher Scientific; Cat. No. A25777) following previously published protocol87 (link). The fold change in gene expression was determined by the ΔΔCt method. GAPDH was used as a housekeeping gene.
Nazeen S., Wang X., Zielinski D., Lam I., Hallacli E., Xu P., Ethier E., Strom R., Zanella C.A., Nithianandam V., Ritter D., Henderson A., Saurat N., Afroz J., Nutter-Upham A., Benyamini H., Copty J., Ravishankar S., Morrow A., Mitchel J., Neavin D., Gupta R., Farbehi N., Grundman J., Myers R.H., Scherzer C.R., Trojanowski J.Q., Van Deerlin V.M., Cooper A.A., Lee E.B., Erlich Y., Lindquist S., Peng J., Geschwind D.H., Powell J., Studer L., Feany M.B., Sunyaev S.R, & Khurana V. (2024). Deep sequencing of proteotoxicity modifier genes uncovers a Presenilin-2/beta-amyloid-actin genetic risk module shared among alpha-synucleinopathies. bioRxiv.
Corresponding Organization : Harvard Stem Cell Institute
Other organizations :
Whitehead Institute for Biomedical Research, Garvan Institute of Medical Research, Massachusetts Institute of Technology, University of California, Los Angeles, Boston University, Institute for Neurodegenerative Disorders, University of Pennsylvania, University of Illinois Urbana-Champaign, Center for Autism and Related Disorders
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