Indirect Calorimetry of Metabolic Rates

To measure metabolic rates, metabolic parameters in the treated mice were analyzed with indirect calorimetry^{43 (link), 44 (link)} using the Comprehensive Laboratory Animal Monitoring System (CLAMS) system (Columbus Instruments, Columbus, OH, USA) at normal temperature (22℃). The mice were individually housed 2–3 days before the experiment for habituation and then fed the same diet (chow or HFD) and water provided ad libitum in clear respiratory chambers (20.5 × 10.5 × 12.5 cm). O₂ consumption, CO₂ production, energy expenditure, heat, and activities of the experimental animals were measured for 24 h. These data were collected with Oxymax for Windows (version 5.40.14, Columbus Instruments) and analyzed with CLAX software (version 2.2.15, Columbus Instruments). Based on the collected data, the respiratory quotient or exchange ratio (CO₂/O₂) and delta-heat values were calculated using the CLAX software.

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Kim D., Lee Y., Kim H.R., Park Y.J., Hwang H., Rhim H., Kang T., Choi C.W., Lee B, & Kim M.S. (2021). Hypothalamic administration of sargahydroquinoic acid elevates peripheral thermogenic signaling and ameliorates high fat diet-induced obesity through the sympathetic nervous system. Scientific Reports, 11, 21315.

Publication 2021

CLAMS) system CLAX software

Diet Energy expenditure Experimental animals Mice Respiratory

Corresponding Organization :

Other organizations : Korea Institute of Brain Science, Korea Institute of Science and Technology, Korean Association Of Science and Technology Studies, Pukyong National University, Korea Institute of Oriental Medicine, Bio-Medical Science (South Korea), Korea University of Science and Technology

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Variable analysis

independent variables

Diet (chow or HFD)

dependent variables

O2 consumption
CO2 production
Energy expenditure
Activities of the experimental animals
Respiratory quotient or exchange ratio (CO2/O2)
Delta-heat values

control variables

Temperature (22°C)
Housing (individually housed in clear respiratory chambers of size 20.5 × 10.5 × 12.5 cm)
Habituation period (2-3 days before the experiment)
Ad libitum access to the same diet (chow or HFD) and water

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