Kidney Immunostaining and Imaging Protocol

Kidney sections and fixed cells were immunostained as described previously [10 (link)]. For immunochemical staining, sections were stained with anti-AQP2 (Cat. No. AQP-002; Alomone Laboratories) antibody. Hematoxylin was used for counter-staining. For immunofluorescence, the following antibodies were used: anti–acetylated (ac)-α-tubulin (Cat. No. T7451; Sigma-Aldrich), anti–Na/K-ATPase (Cat. No. ab76020; Abcam), anti-AQP1 (Cat. No. AQP-001; Alomone Laboratories), and anti-AQP2 (Cat. No. AQP-002). To detect cell nuclei, DAPI was applied to samples. Images were captured using a Leica microscope (DM2500; Wetzlar).

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Kong M.J., Han S.J., Seu S.Y., Han K.H., Lipschutz J.H, & Park K.M. (2023). Shortening of primary cilia length is associated with urine concentration in the kidneys. Kidney Research and Clinical Practice, 42(3), 312-324.

Publication 2023

anti–Na/K-ATPase anti-AQP1 anti-AQP2 Leica microscope (DM2500

Antibodies Antibody Aqp1 Cell nuclei Cells Dapi Hematoxylin Immunofluorescence Kidney Microscope Na k atpase α tubulin

Corresponding Organization :

Other organizations : Kyungpook National University, Ewha Womans University, Medical University of South Carolina

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Variable analysis

independent variables

Immunostaining with anti-AQP2 antibody
Immunofluorescence staining with anti–acetylated (ac)-α-tubulin, anti–Na/K-ATPase, anti-AQP1, and anti-AQP2 antibodies

dependent variables

Immunochemical staining results
Immunofluorescence staining results

control variables

Hematoxylin counter-staining
DAPI staining to detect cell nuclei
Imaging using a Leica microscope (DM2500)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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