Comprehensive TAG Profiling of Berry Seed Oils

The conditions of UPC²-Q TOF MS were set according to a previously reported laboratory procedure [26 (link),27 (link),28 (link),29 (link)]. Waters Acquity UPC² system (Milford, MA, USA) equipped with an Acquity UPC² BEH HSS C18 column (150 mm × 3.0 mm i.d.; 1.7 µm) was utilized for the separation of TAGs. All the other instrument conditions were similar as previous reported method. A Waters 1525 single pump was used as the compensated pump, and pumped 0.3 mL/min of 0.1% ammonium formate in methanol into the MS source. The TAG compositions of the berry seed oils were analyzed and semi-quantified with a Waters Xevo-G2 Q-TOF MS system as previously described. The ion mode, capillary voltage, cone voltage, temperatures of source and desolvation, as well as other parameters were similar as our previous publications. The collision energy was 6 eV in MS1, and information of fragment ions was collected in the MS2 mode and the collision energy was 35 eV, with the scan time set at 0.2/s.