CD34+ Cell Isolation from CML Patients

Blood samples were collected from CML patients at chronic phase in Zhongda Hospital Southeast University, Nanjing, China. Leukopheresis samples were underway for CD34⁺ cells separation with CliniMACS (Miltenyi Biotech, Germany). CD34⁺ cells were selected by using anti-CD34 magnetic beads in a magnetic activated cell sorter system (Miltenyi Biotec). All subjects signed an informed consent form. The procedure of cell collection from patients conformed to guidelines in the Declaration of Helsinki, and was approved by the Institutional Review Board of Zhongda Hospital Southeast University.

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Xu X., Ji S., Chen Y., Xia S., Li Y., Chen L., Li Y., Zhang F., Zhang Z, & Zheng S. (2023). Induction of DNMT1-dependent demethylation of SHP-1 by the natural flavonoid compound Baicalein overcame Imatinib-resistance in CML CD34+ cells. Cell Communication and Signaling : CCS, 21, 47.

Publication 2023

CliniMACS magnetic activated cell sorter system

Blood Cells separation Institutional review board Leukopheresis Patients

Corresponding Organization : Nanjing University of Chinese Medicine

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Variable analysis

independent variables

Cell collection method (Leukopheresis for CD34+ cells separation)

dependent variables

CD34+ cell population

control variables

Blood samples collected from CML patients at chronic phase
CD34+ cells selected using anti-CD34 magnetic beads in a magnetic activated cell sorter system
Informed consent obtained from all subjects
Cell collection procedure conformed to Declaration of Helsinki guidelines
Cell collection procedure approved by Institutional Review Board

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