Fecal Microbiota Transplant for rCDI in IBD

All patients recruited into this study suffered from rCDI with underlying IBD and failed to eradicate the infection, despite several rounds of antibiotic therapies as presented in our previous work (Gholam-Mostafaei et al., 2021 (link)). Healthy stool donors were rigorously screened and included genetically related, patient-oriented first-degree and third-degree relatives, who donated freshly passed fecal materials on the day of transplantation, which was rapidly processed within 6 h of defecation. None of the donor fecal specimens were frozen or banked. Stool samples from the recipients were collected prior to FMT (pre-FMT) and at day 28 (post-FMT), and were stored in aliquots at –80°C until further analysis (Figure 1). FMT procedure was carried out via colonoscopy, and the fecal suspension was infused into the terminal ileum or cecum of the patients as previously described (Gholam-Mostafaei et al., 2021 (link)).

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Gholam-Mostafaei F.S., Azimirad M., Naseri K., Nabavi-Rad A., Asadzadeh Aghdaei H., Shahrokh S., Ebrahimi Daryani N., Yadegar A, & Zali M.R. (2023). Intestinal microbiota changes pre- and post-fecal microbiota transplantation for treatment of recurrent Clostridioides difficile infection among Iranian patients with concurrent inflammatory bowel disease. Frontiers in Microbiology, 14, 1147945.

Publication 2023

Antibiotic Cecum Colonoscopy Defecation Donor Fecal Frozen Ileum Infection Patients

Corresponding Organization : Shahid Beheshti University of Medical Sciences

Other organizations : Tehran University of Medical Sciences

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Variable analysis

independent variables

Fecal Microbiota Transplantation (FMT)

dependent variables

Recurrence of Clostridioides difficile infection (rCDI) in patients with underlying inflammatory bowel disease (IBD)
Microbial composition in stool samples before and after FMT

control variables

Healthy stool donors who were rigorously screened and included genetically related, patient-oriented first-degree and third-degree relatives
Freshly passed fecal materials from donors on the day of transplantation, rapidly processed within 6 h of defecation
Stool samples from recipients collected prior to FMT (pre-FMT) and at day 28 (post-FMT), stored in aliquots at –80°C

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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