hiPSCs were cultured on irradiated DR4 mouse embryonic fibroblast feeders in the following cell culture media: DMEM/F12 (1:1) (Invitrogen) containing 20% KnockOut Serum (Invitrogen), 1 mM non-essential amino acids (Invitrogen, 1:100), GlutaMax (Invitrogen, 1:100), 0.1 nM β-mercaptoethanol (Sigma-Aldrich), 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen) and 10–15 ng/ml FGF2 (R&D Systems).
The lines/clones of hiPSC used in this study were validated using standardized methods as previously shown32 (link),41 (link). Cultures are regularly tested and maintained mycoplasma free. A total of seven iPSC lines (6593-8, 8343-3, 1804-5, NH1-1, 8858-1, 8858-3, 8858-C) derived from five subjects were used for experiments. Approval for this study was obtained from the Stanford IRB Panel and informed consent was obtained from all subjects.
The lines/clones of hiPSC used in this study were validated using standardized methods as previously shown32 (link),41 (link). Cultures are regularly tested and maintained mycoplasma free. A total of seven iPSC lines (6593-8, 8343-3, 1804-5, NH1-1, 8858-1, 8858-3, 8858-C) derived from five subjects were used for experiments. Approval for this study was obtained from the Stanford IRB Panel and informed consent was obtained from all subjects.
