Targeted Metabolomics Analysis by HILIC-MS

Analytical separation and quantification were performed on a Dionex^TM UltiMate^TM 3000 system (Thermo Fisher Scientific, Dreieich, Germany) equipped with a SeQuant ZIC-HILIC column (5 μm, 2.1 × 150 mm, SeQuant with guard column, Merck, Darmstadt, Germany). Mass spectra were recorded on a Q-Exactive^TM Plus Orbitrap mass spectrometer (Thermo Fisher Scientific, Dreieich, Germany). The electrospray ionization conditions are given in [7 (link)]. The column temperature was set to 25 °C. Mass measurements were performed in the HESI-positive mode, full scan mode from 75 to 200 m/z, at a resolution of 70,000. For qualitative MS/MS analysis, the collision energy was set to 35 V and data were collected in DIA (Data Independent Acquisition) mode.

Free full text: Click here

Fenizia S., Weissflog J, & Pohnert G. (2021). Cysteinolic Acid Is a Widely Distributed Compatible Solute of Marine Microalgae. Marine Drugs, 19(12), 683.

Publication 2021

DionexTM UltiMateTM 3000 system SeQuant ZIC-HILIC column Q-ExactiveTM Plus Orbitrap mass spectrometer

Mass spectra Ms ms Scan

Corresponding Organization : Max Planck Institute for Chemical Ecology

Top 5 similar protocols

Variable analysis

independent variables

Analytical separation and quantification were performed on a Dionex UltiMate 3000 system
Mass spectra were recorded on a Q-Exactive Plus Orbitrap mass spectrometer

dependent variables

Analytical separation and quantification
Mass spectra

control variables

Column temperature was set to 25 °C
Mass measurements were performed in the HESI-positive mode, full scan mode from 75 to 200 m/z, at a resolution of 70,000
For qualitative MS/MS analysis, the collision energy was set to 35 V and data were collected in DIA (Data Independent Acquisition) mode

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!