Grapevine Variety Identification via Microsatellite Analysis

For variety identification and confirmation, eight vines were sampled. DNA was extracted using the peqGOLD Plant DNA mini kit (PEQLAB Biotechnologie GmbH, Erlangen, Germany) according to the manufacturer's instructions. Nine microsatellite (simple sequence repeats, SSR) primers recommended for routine variety distinction of grapevine were used (15 ). Polymerase chain reaction (PCR) amplifications were carried out in an Applied Biosystems Veriti™ thermal cycler (Thermo Fisher Scientific, Foster City, CA, USA). The list and information about the used primers, as well as detailed information on multiplex PCR reactions performed, are described by Žulj Mihaljević et al. (4 (link)). Amplified products were separated using an Applied Biosystems 3130 genetic analyzer (Thermo Fisher Scientific) with GeneScan™ 500 LIZ^® size standard. Sizing of the fragments was performed using GeneMapper v. 4.0 software (15 ). The obtained SSR profiles were compared to internal microsatellite database comprising profiles on 9 common loci from European Vitis database (16 ) as well as published SSR profiles from other research (4 (link)). Data were standardized and compared as described previously (4 (link)).