Cell Counting Using Scepter Device

To determine cell number, cells were washed with PBS and dissociated by trypsin. Cells were then resuspended with DMEM containing 10% FBS and cell number was measured using a cell counter (Millipore, Billerica, MA, USA). Cell number counted by a Scepter cell counter was based on Coulter principle of impedance-based particle detection [54 (link)]. Cells ranging in diameter between 10 to 22 μm were counted as surviving cells [55 (link)].

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Yeom M., Lee H., Shin S., Park D, & Jung E. (2018). PER, a Circadian Clock Component, Mediates the Suppression of MMP-1 Expression in HaCaT Keratinocytes by cAMP. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 745.

Publication 2018

cell counter

Cells Trypsin

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cell number

control variables

Cells were washed with PBS
Cells were dissociated by trypsin
Cells were resuspended with DMEM containing 10% FBS
Cell number was measured using a cell counter (Millipore, Billerica, MA, USA)
Cells ranging in diameter between 10 to 22 μm were counted as surviving cells

controls

No positive or negative controls were explicitly mentioned in the provided information.

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