Blood and PBMC Collection Protocol

Blood samples were taken directly from the jugular vein into Venojet glass tubes, without anticoagulant on days 0 (naïve), 7, 21, 28, 42 (pre-challenge), 3, 5, 7, 11 and 13 (post-challenge) and allowed to clot overnight at 4°C. Serum was obtained by centrifugation at 3000 rpm for 10 minutes (min) at 4°C, aliquoted and stored at −80°C until use. PBMCs were obtained from total blood collected in EDTA and purified by Ficoll cushion (GE Healthcare) purification as described previously [28] (link).

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Rojas J.M., Moreno H., Valcárcel F., Peña L., Sevilla N, & Martín V. (2014). Vaccination with Recombinant Adenoviruses Expressing the Peste des Petits Ruminants Virus F or H Proteins Overcomes Viral Immunosuppression and Induces Protective Immunity against PPRV Challenge in Sheep. PLoS ONE, 9(7), e101226.

Publication 2014

Ficoll cushion

Anticoagulant Blood Centrifugation Clot Edta Ficoll Jugular vein Serum

Corresponding Organization :

Other organizations : Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

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Variable analysis

independent variables

Days of sample collection (0, 7, 21, 28, 42 (pre-challenge), 3, 5, 7, 11 and 13 (post-challenge))

dependent variables

Serum samples
PBMC samples

control variables

Blood samples were taken directly from the jugular vein
Blood samples were collected into Venojet glass tubes without anticoagulant
Blood samples were allowed to clot overnight at 4°C
Serum was obtained by centrifugation at 3000 rpm for 10 minutes at 4°C
PBMCs were obtained from total blood collected in EDTA and purified by Ficoll cushion (GE Healthcare) purification

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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