Western Blotting for EMT Markers

Protein extraction and Western blotting were conducted as previously described 19 (link), 21 (link), 22 (link). The antibodies used were affinity-purified rabbit anti-INHBB polyclonal antibody (Abcam, Cambridge, UK), mouse anti-GAPDH polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), rabbit anti-E-cadherin monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA), and rabbit anti-Zonula occludens-1 (Zo-1) monoclonal antibody and rabbit anti-snail family transcriptional repressor 1 (Snail) monoclonal antibody (Cell Signaling Technology).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kita A., Kasamatsu A., Nakashima D., Endo-Sakamoto Y., Ishida S., Shimizu T., Kimura Y., Miyamoto I., Yoshimura S., Shiiba M., Tanzawa H, & Uzawa K. (2017). Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis. Journal of Cancer, 8(11), 2033-2041.

Publication 2017

mouse anti-GAPDH polyclonal antibody rabbit anti-E-cadherin monoclonal antibody

Anti antibody Antibodies Cadherin Gapdh Inhbb Monoclonal antibody Mouse Protein Rabbit Snail Transcriptional Zonula occludens

Corresponding Organization :

Other organizations : Chiba University, Chiba University Hospital, National Defense Medical College

Top 5 similar protocols

Variable analysis

independent variables

Protein extraction and Western blotting methods

dependent variables

Expression levels of INHBB, GAPDH, E-cadherin, Zonula occludens-1 (Zo-1), and Snail family transcriptional repressor 1 (Snail)

control variables

Unspecified

controls

Positive controls: Unspecified
Negative controls: Unspecified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!