Neuromolecular Synthesis and Pharmacological Targets

NMP-7 was synthesized at the Core Laboratory for Neuromolecular Production at the University of Montana. NMP-7 was dissolved in DMSO (to a maximum of 5%) and PBS. Selective CB₁ antagonist AM281, irreversible inhibitor of monoacylglycerol lipase JZL184 [32 (link), 33 (link)], selective CB₂ antagonist AM630, and the irreversible inhibitor of fatty acid amide hydrolase URB597 [34 (link)] were provided by Cayman Chemical, and dissolved in phosphate buffered saline (PBS) and dimethyl sulfoxide (DMSO) to 5%. Complete Freund’s Adjuvant (CFA), o-Dianisidine and DMSO were supplied by Sigma Aldrich. The myeloperoxidase (MPO) assay standard was supplied by Calbiochem (EMD Millipore).