The production of NO was estimated by measuring the amount of nitrite, a stable metabolite of NO, using the Griess reagent as described [14 (link)]. After THC-pretreated BV2 microglial cells were stimulated with LPS in 12-well plates for 24 hr, and then 100 µl of the cell culture media was mixed with an equal volume of Griess reagent. Light absorbance was read at 540 nm. The results were expressed as a percentage of released NO from LPS-stimulated BV2 cells. To prepare a standard curve, sodium nitrite was used to prepare a standard curve.
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Quantifying Nitric Oxide in Microglial Cells
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Other organizations : Kangwon National University, Seoul National University, National Institute of Food and Drug Safety Evaluation
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Variable analysis
independent variables
- Treatment with THC (Tetrahydrocannabinol)
- Production of NO (Nitric Oxide) measured by the amount of nitrite, a stable metabolite of NO, using the Griess reagent
- BV2 microglial cells
- Stimulation with LPS (Lipopolysaccharide) for 24 hours
- Positive control: LPS-stimulated BV2 cells
- Standard curve: Sodium nitrite used to prepare a standard curve
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