Cranial Window Implantation for In Vivo Imaging

The procedure was done as in [22 (link)], Carprofen (Pfizer 15 ug/25 g mouse) analgesia was administered subcutaneously prior to surgery and then daily for the next 4 days. Mice were anesthetized with Isoflurane (5% for induction, 1–2% thereafter), the scalp and connective tissue were removed, and the skull was covered with VetBond. An aluminum metal bar with 2 traded holes was attached to the skull with black Dental Acrylic. A 3 mm diameter craniotomy was done above part of the primary somatosensory cortex (S1) known as the barrel cortex (from Bregma: rostral −1.5, lateral 3 mm). A custom-made 3mm coverglass (Bellco Glass) was placed and sealed with VetBond cyanoacrylate glue. The dry glue was covered with Dental Acrylic. One ml Ringer solution was given subcutaneous after the surgery. During the surgery, and until full recovery, the mouse temperature was kept at 37°C using a heated plate and a rectal temperature sensor.