Protocol for 68Ga-PSMA-11 PET/CT Imaging

⁶⁸Ga-PSMA-11 was prepared in house as previously described by our group [13 (link)]. An average of 1.85 MBq per kilogram ⁶⁸Ga-PSMA-11 was injected intravenously. The median injected activity was 133 MBq (range, 59.2–247.9 MBq). Radiochemical purity of injected radiopharmaceutical was above 95% in all participants. Whole-body PET/CT imaging from vertex to mid-thigh was performed at 60 min post-injection (range, 60–70 min). A low-dose non-diagnostic CT without contrast was performed for anatomical localization and attenuation correction. There was no special preparation prior to imaging. Patients were requested to void fully just prior to imaging beginning. All patients were imaged on a Biograph 40 Truepoint PET/CT scanner (Siemens Medical Solution, Malvern, PA, USA). Image reconstruction was completed with an ordered subset expectation maximization iterative reconstruction algorithm (four iterations, eight subsets). A Gaussian filter was applied at 5.0 mm FWHM.