Antibody Validation and Boron Compound Synthesis

Anti-SREBP1 (2A4; Santa Cruz Biotechnology, Inc.), anti-FAS (Cell Signaling Technology, Inc.), anti–Flag M2 (Sigma-Aldrich), anti–β-actin (Sigma-Aldrich), and anti–β-tubulin (Life Technologies) antibodies were purchased in this study. The boron-containing compounds BF175 and BF62 were synthesized and purified according to the method we reported previously (16 (link)).

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Zhao X., X.i.a.o.l.i., Zong H., Abdulla A., Yang E.S., Wang Q., Ji J.Y., Pessin J.E., Das B.C, & Yang F. (2014). Inhibition of SREBP Transcriptional Activity by a Boron-Containing Compound Improves Lipid Homeostasis in Diet-Induced Obesity. Diabetes, 63(7), 2464-2473.

Publication 2014

Anti-SREBP1 (2A4; anti-FAS anti–Flag M2 anti–β-actin anti–β-tubulin

Actin Antibodies Boron compounds Srebp1 Tubulin

Corresponding Organization : Albert Einstein College of Medicine

Other organizations : Renji Hospital, Texas A&M Health Science Center, University of Kansas Medical Center

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Variable analysis

independent variables

BF175

dependent variables

SREBP1 expression
FAS expression
Flag M2 expression
β-actin expression
β-tubulin expression

control variables

Anti-SREBP1 (2A4; Santa Cruz Biotechnology, Inc.) antibody
Anti-FAS (Cell Signaling Technology, Inc.) antibody
Anti–Flag M2 (Sigma-Aldrich) antibody
Anti–β-actin (Sigma-Aldrich) antibody
Anti–β-tubulin (Life Technologies) antibody

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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