Quantifying Lung Tumor Metastasis

Histologic sections of paraffin-embedded lung and thymus tissues (5 μm) were stained with hematoxylin and eosin (HE) and imaged using the digital slide scanner NanoZoomer 2.0-HT system (0.46 μm/pixel (20X) scanning resolution) (Hamamatsu Photonics, Hamamatsu City, Japan). Metastatic dissemination to lungs was evaluated on five HE-stained sections (separated each other by 50 μm) per mouse lung by measuring tumor area in lungs and reporting it to the total area of lung tissue analyzed. Quantification of lung tumor area was performed as described previously [48 (link)].

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Gérard C., Hubeau C., Carnet O., Bellefroid M., Sounni N.E., Blacher S., Bendavid G., Moser M., Fässler R., Noel A., Cataldo D, & Rocks N. (2018). Microenvironment-derived ADAM28 prevents cancer dissemination. Oncotarget, 9(98), 37185-37199.

Publication 2018

NanoZoomer 2.0-HT system

Embedded paraffin Eosin Lung Mouse Scanner Thymus Tissue Tumor of lung

Corresponding Organization :

Other organizations : University of Liège, Max Planck Institute of Biochemistry

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Variable analysis

independent variables

Presence of metastatic dissemination to lungs

dependent variables

Tumor area in lungs
Total area of lung tissue analyzed

control variables

Histologic sections of paraffin-embedded lung and thymus tissues
Tissue sections with a thickness of 5 μm
Staining with hematoxylin and eosin (HE)
Imaging using the digital slide scanner NanoZoomer 2.0-HT system with a scanning resolution of 0.46 μm/pixel (20X)

controls

Five HE-stained sections separated each other by 50 μm per mouse lung were analyzed

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