Phenotypic Characterization of Bone Marrow-Derived Mesenchymal Stem Cells

APCs were stained for surface antigen expression using combinations of the following antibodies to confirm typical phenotype: CD105 (Life Technologies), CD90, CD73, PDGFRβ (Biolegend), CD44 (eBioscience), CD31 (BD Biosciences), CD34, and CD45 (Miltenyi)^{5 (link), 10 (link)}. Analysis was performed using a FACS Canto II flow cytometer and FACS Diva software (both BD Biosciences). BM-MSCs antigenic characterization was also routinely performed by flow cytometry. Primers and antibodies are listed in Supplementary Tables VI and VII, respectively.

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Riu F., Slater S.C., Garcia E.J., Rodriguez-Arabaolaza I., Alvino V., Avolio E., Mangialardi G., Cordaro A., Satchell S., Zebele C., Caporali A., Angelini G, & Madeddu P. (2017). The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling. Scientific Reports, 7, 5443.

Publication 2017

CD105 PDGFRβ FACS Canto II flow cytometer FACS Diva software

Antibodies Antigenic Apcs Cd44 Cd73 Cd90 Flow cytometry Pdgfrβ Phenotype Primers Surface antigen

Corresponding Organization :

Other organizations : Bristol Royal Infirmary, University of Bristol, The Queen's Medical Research Institute

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Variable analysis

independent variables

Combinations of the following antibodies: CD105, CD90, CD73, PDGFRβ, CD44, CD31, CD34, and CD45

dependent variables

Surface antigen expression of APCs

control variables

Not explicitly mentioned

controls

BM-MSCs antigenic characterization was also routinely performed by flow cytometry as a control

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