A clinical isolate of B. pseudomallei from a melioidosis case in University Malaya Medical Center (UMMC) isolated as previously described was used in the study [47 (link)]. The isolate, when cultured on agar medium at 37°C, was found to differentiate into two colony morphotypes, OB (WT, INSDC: APLK00000000.1) and OS (SCV, INSDC: APLL00000000.1). OB and OS were characterized using a commercial analytical profile index API 20NE (BioMėrieux) test and an in house PCR assay specific for B. pseudomallei [48 (link)]. The two morphotypes were cultured on nutrient agar and a single colony of each morphotype was inoculated into Luria-Bertani (LB) broth (Becton Dickinson, Franklin Lakes, New Jersey, USA) at 37°C overnight in a shaker incubator at 200 revolutions per minute (rpm). Following culture, glycerol (Acros Organics, Geel, Belgium) with a final concentration of 30% (v/v) was added to the LB cultures and stored at -80°C as a stock culture for the entire duration of the study.
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