Confluent MCF-7 cell monolayers were serum-starved overnight, scratched with a p-200 micropipette tip, and treated with 100 ng/ml epidermal growth factor (EGF) for 16 hours. For live imaging, phase micrographs were taken with a Nikon TMS phase Microscope equipped with a Nikon Coolpix digital camera (Nikon, Mississauga, ON, Canada) to capture the migratory infilling of the scratch/wound. Wound areas were quantified using CellSens Software (Olympus, Richmond Hill, ON, Canada).
In some experiments the scratched monolayers were treated with either dimethylsulfoxide (DMSO; vehicle control) or the ezrin inhibitor NSC668394 (Millipore, Etobicoke, ON, Canada [39 (link)]) diluted to 10 μM in DMSO.
In some experiments the scratched monolayers were treated with either dimethylsulfoxide (DMSO; vehicle control) or the ezrin inhibitor NSC668394 (Millipore, Etobicoke, ON, Canada [39 (link)]) diluted to 10 μM in DMSO.
Free full text: Click here
