Identification of Lipid Compounds by GC-MS

A standard mixture containing 39 FAMEs (GLC 566, Nu Chek Prep Inc., MA, United States) or standardized AcOMEs obtained in the previous section (Study II) were analyzed by GC–MS chromatography (Agilent 7890B/5977A, Agilent Technologies Inc., CA, United States) to identify the lipids. A previously developed method was used (Castillo et al., 2020 (link)). Having as reference fragmentation patterns of FAMEs (Jabbar et al., 2014 ; Busta et al., 2016 (link)) and Mclafferty rearrangement characteristic of esters (McLafferty, 1956 (link); Weiler, 1972 (link)), the expected fragmentation patterns of each of the free AcO-acetogenins reported here (based on their structure) were matched to their observed mass spectra (Supplementary Figure 2).