Feeder-free culture of iPSC line 201B7

The validated iPSC line 201B7 was purchased from Riken Cell bank (Tsukuba, Japan) and transferred from on-feeder to feeder-free conditions in our laboratory. We cultured the iPSC lines according to a previously described method (Nakagawa et al., 2014 (link)). In brief, the culture plates were precoated with iMatrix-511 (0.5 μg/cm²), and the iPSC were maintained in Stem Fit medium (Ajinomoto) with penicillin (100 units/mL) and streptomycin (100 μg/mL; Life Technologies, MA, USA) at 37°C with 5% CO₂. The medium was changed every other day and passaged every 7-10 days using 0.5× TrypLE Select (1× TrypLE Select diluted 1:1 with 0.5 mM EDTA/PBS [-]; Life Technologies) and Rho-associated kinase (Rock) inhibitor (Y-27632; WAKO, Osaka, Japan).