Anti-angiogenic Effects of Bac I and II

A tube formation assay was performed to assess the anti-angiogenic effect of bac I and II, either alone or in combination, on 2H-11 and HUVEC, as previously described [13 (link),58 (link)]. Briefly, 96-well angiogenesis μ-plates (Ibidi, Martinsried, Germany) were prepared by coating wells with Matrigel (Corning), according to the manufacturer’s instructions. In the respective complete medium, 2H-11 and HUVEC were resuspended, supplemented with either vehicle or bac I and/or II, and were plated at 1.5 × 10⁴ cells per well. After 2-h incubation, the number of loops formed was counted.

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Tomita Y., Smith E., Palethorpe H.M., Nakhjavani M., Yeo K.K., Townsend A.R., Price T.J., Yool A.J, & Hardingham J.E. (2021). In Vitro Synergistic Inhibition of HT-29 Proliferation and 2H-11 and HUVEC Tubulogenesis by Bacopaside I and II Is Associated with Ca2+ Flux and Loss of Plasma Membrane Integrity. Pharmaceuticals, 14(5), 436.

Publication 2021

96-well angiogenesis μ-plates Matrigel

Angiogenesis Anti angiogenic effect Assay Cells Matrigel

Corresponding Organization : University of Adelaide

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Variable analysis

independent variables

Bac I
Bac II
Combination of bac I and bac II

dependent variables

Number of loops formed

control variables

Cell lines (2H-11 and HUVEC)
Matrigel coating
Incubation time (2 hours)
Cell density (1.5 × 10^4 cells per well)

controls

Vehicle control

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