PEDV RNA Detection in Rectal Swabs

Total nucleic acids were extracted from all rectal swabs using the MagMax™ Pathogen RNA Kit (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) on an automated nucleic acid extraction system (Thermo Scientific Kingfisher^® Flex, Thermo Fisher Scientific, Pittsburgh, PA, USA) according to the instructions of the manufacturer. All RNA extracts were tested for the presence of PEDV RNA by a quantitative real-time PCR [19 (link)]. Samples were considered negative when no signal was observed within 40 amplification cycles.

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Opriessnig T., Gerber P.F., Shen H., de Castro A.M., Zhang J., Chen Q, & Halbur P. (2017). Evaluation of the efficacy of a commercial inactivated genogroup 2b-based porcine epidemic diarrhea virus (PEDV) vaccine and experimental live genogroup 1b exposure against 2b challenge. Veterinary Research, 48, 69.

Publication 2017

MagMax™ Pathogen RNA Kit Thermo Scientific Kingfisher® Flex

Nucleic acid Pathogen Pedv Quantitative real time pcr Rectal

Corresponding Organization :

Other organizations : Roslin Institute, University of Edinburgh, Iowa State University

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Variable analysis

independent variables

Nucleic acid extraction method (MagMax™ Pathogen RNA Kit)

dependent variables

Presence of PEDV RNA (measured by quantitative real-time PCR)

control variables

Automated nucleic acid extraction system (Thermo Scientific Kingfisher® Flex)
Amplification cycles (samples considered negative when no signal was observed within 40 cycles)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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