Transthoracic echocardiography was performed assessed pre-myocardial infarction (base-line) and at day 28 post-treatment by the Vevo-770 echocardiography machine (VisualSonics, Fujifilm, Amsterdam, Netherlands) under isoflurane inhalation administered via a nose cone as we have previously described [[14] , [15] (link), [16] (link),31 (link)]. LV end-diastolic (LVEDD) and end-systolic (LVESD) dimensions, under stable, consistent heart rate, were measured using M mode. Left ventricular ejection fraction (LVEF) was calculated from the data obtained with 2-dimensional tracing. All data were collected blind from at least 3–5 different measurements.
Hemodynamic parameters were measured by using cardiac catheterization (SPR-320 and PVAN3.2; Millar Instruments, USA) as we have previously described [[14] , [15] (link), [16] (link),31 (link)]. Briefly, under general anesthesia using isoflurane inhalation with a nose cone, the catheter was inserted into the left ventricular cavity through the right common carotid artery. Intra-LV pressure signals were measured with a transducer and conditioner (MPVS-300; Millar Instruments) and digitally recorded with a data acquisition system (PowerLab 8/30; AD Instruments, Oxford, UK). All data were collected from at least 5 different measurements in a blinded manner.
Hemodynamic parameters were measured by using cardiac catheterization (SPR-320 and PVAN3.2; Millar Instruments, USA) as we have previously described [[14] , [15] (link), [16] (link),31 (link)]. Briefly, under general anesthesia using isoflurane inhalation with a nose cone, the catheter was inserted into the left ventricular cavity through the right common carotid artery. Intra-LV pressure signals were measured with a transducer and conditioner (MPVS-300; Millar Instruments) and digitally recorded with a data acquisition system (PowerLab 8/30; AD Instruments, Oxford, UK). All data were collected from at least 5 different measurements in a blinded manner.
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