Transgenic Caenorhabditis elegans Strain Generation

Strains were generated by Mos-1 single copy insertion following published protocols [38 (link)], by micro-injection of plasmid DNA into the gonads of young adults of strain EG6699. Mos-1 insertions were verified by PCR with LongAmp Taq polymerase (New England Biolabs) followed by Sanger sequencing. The Aβ42 plasmid for overexpression was micro-injected into the gonads of young adults of strain AX204 together with a plasmid encoding cc::RFP, and transgenic offspring was selected based on RFP expression in the coelomocytes.

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Sinnige T., Ciryam P., Casford S., Dobson C.M., de Bono M, & Vendruscolo M. (2019). Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates the associated behavioural response. PLoS ONE, 14(5), e0217746.

Publication 2019

LongAmp Taq polymerase

Gonads Insertions Plasmid Strain Taq polymerase Transgenic Young adults

Corresponding Organization : Medical Research Council

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Variable analysis

independent variables

Mos-1 single copy insertion
Microinjection of Aβ42 plasmid for overexpression

dependent variables

Presence of Mos-1 insertions (verified by PCR and Sanger sequencing)
Transgenic offspring expression of cc::RFP

control variables

Young adults of strain EG6699 for Mos-1 insertion
Young adults of strain AX204 for Aβ42 overexpression

controls

Positive control: Microinjection of plasmid DNA into the gonads of young adults
Negative control: Not explicitly mentioned

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