Dietary Fat Composition and Metabolism

The first animal experiment was performed using 45 lean male mice, 8 weeks old. After one week of acclimatization, body mass composition was determined and mice were divided into groups with similar body weight (25 ± 0.7 g), fat mass (2.5 ± 0.2 g) and lean mass (18.5 ± 0.6 g). The mice were fed the experimental diets (Table 1) ad libitum, including a LF diet, a high-fat/high-sucrose (HF/HS) diet and HF/HP diets based on either casein, cod or pork for 12 weeks (n = 9). All animals were subjected to an oral glucose tolerance test (OGTT) and an insulin tolerance test (ITT) after 10 and 11 weeks, respectively, as further described in Section 2.5. In week 6, total feces was collected during 7 days from all the individual cages, weighed and analyzed for total nitrogen and total fat content as previously described [29 (link)]. Apparent digestibility of nitrogen and fat were determined using the total fat and nitrogen content in the collected feces, in addition to the total dietary fat and nitrogen intake during the 7 days of feces collection.