Facile Nanoparticle Generation via CIJ

NPs were generated using a FNP process (Figure 1A) as previously described [10 (link)]. Briefly, NPs were generated using a three-inlet, confined impinging jet (CIJ) FNP device. The first two inlet inputs contained distilled, deionized (DDI) water. The third inlet contained a solution of 5 mg/mL PEG_3K-b-PLGA_20K containing 90 mol% unlabeled PEG_3K-b-PLGA_20K (Polyscitech) and 10 mol% maleimide-PEG_3K-b-PLGA_20K (Polyscitech) dissolved in acetonitrile. NPs of the defined size were generated by modulating volumetric flow rates of the three inlets using a NE-4000 Programmable 2 Channel Syringe Pump (New Era Pump Systems). The NPs generated through the device were collected in a water bath such that ≤10% v/v was acetonitrile. The collected NPs were dialyzed in a 3.5-kDa dialysis membrane (Repligen) at 4 °C against 4 L of deionized water with dialysate changes every six hours.