Deciphering LATS2, SIAH2, CTGF, YAP, and α-SMA Interplay

Tissue sections were stained with antibodies to LATS2 (Novus, USA), SIAH2 (Novus, USA), CTGF (Santa-Cruz, USA), YAP (CST, USA) and α-SMA (Protein-tech, USA) for 14–16 h at 4℃ and followed by staining with Alexa-488 conjugated anti-Rabbit and Alexa-595 conjugated anti-Mouse respectively. 4’, 6-diamidino-2- phenylindole dihydrochloride (DAPI) was used to color nuclei. Confocal images were recorded by FV3000 confocal microscope (Olympus, Japan).

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Cheng C., Yang H., Yang C., Xie J., Wang J., Cheng L., He J., Li H., Yuan H., Guo F., Li M, & Liu S. (2024). LATS2 degradation promoted fibrosis damage and rescued by vitamin K3 in lupus nephritis. Arthritis Research & Therapy, 26, 64.

Publication 2024

LATS2 SIAH2 α-SMA FV3000 confocal microscope

Corresponding Organization :

Other organizations : Southern Medical University, First Affiliated Hospital of Jinan University, Ministry of Education of the People's Republic of China

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Variable analysis

independent variables

Antibodies used for staining: LATS2 (Novus, USA), SIAH2 (Novus, USA), CTGF (Santa-Cruz, USA), YAP (CST, USA), α-SMA (Protein-tech, USA)

dependent variables

Protein expression levels of LATS2, SIAH2, CTGF, YAP, and α-SMA as measured by confocal microscopy

control variables

Incubation time: 14-16 hours
Incubation temperature: 4°C
Staining with Alexa-488 conjugated anti-Rabbit and Alexa-595 conjugated anti-Mouse
DAPI staining for nuclei
Confocal microscope used: FV3000 (Olympus, Japan)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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