Noninvasive cell growth
and morphology analyses of live cells were performed using 10×
and 20× phase contrast objectives in a BioStation CT using the
automatic Z-focus.40 After attachment,
BEAS-2B cells were treated with Puff EC fluids (0.1–10%) or
with WS-23 (0.045–4.5 mg/mL) solutions dissolved in cell culture
medium. Images were taken every 2 h for 48 h to collect time-lapse
data for analysis. Evaluation of BEAS-2B growth and morphology was
compared in control and treated groups using Nikon CL Quant software
(Melville, NY).40 −42 (link) Data from the treated groups were normalized to untreated
controls.
and morphology analyses of live cells were performed using 10×
and 20× phase contrast objectives in a BioStation CT using the
automatic Z-focus.40 After attachment,
BEAS-2B cells were treated with Puff EC fluids (0.1–10%) or
with WS-23 (0.045–4.5 mg/mL) solutions dissolved in cell culture
medium. Images were taken every 2 h for 48 h to collect time-lapse
data for analysis. Evaluation of BEAS-2B growth and morphology was
compared in control and treated groups using Nikon CL Quant software
(Melville, NY).40 −42 (link) Data from the treated groups were normalized to untreated
controls.
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