Repetitive Sequence-Based DNA Fingerprinting

The isolation of genomic DNA was extracted by the CTAB method as described previously [37 (link)]. Repetitive sequence-based polymerase chain reaction (rep-PCR) used the (GTG)₅ primer (5′-GTGGTGGTGGTGGTG-3′) [22 (link)]. After electrophoresis, Gelcompar II version 6.6 (Applied Maths, Sint-Matenslatem, Belgium) was used to analyze the images of the amplicon fingerprint. The software analyzed the rep-PCR profiles and made use of Pearson correlation analysis to read the banding patterns, and a dendrogram was created by the unweighted pair group method with arithmetic averages [28 (link)]. The raw data for the REP-PCR fingerprint was an additional figure file shows this in more detail (see Additional file 2).

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Kan Z., Luo B., Cai J., Zhang Y., Tian F, & Ni Y. (2020). Genotyping and plant-derived glycan utilization analysis of Bifidobacterium strains from mother-infant pairs. BMC Microbiology, 20, 277.

Publication 2020

Gelcompar II version 6.6

Ctab Electrophoresis Genomic Isolation Primer Repetitive sequence

Corresponding Organization :

Other organizations : Shihezi University, Jiangnan University

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Variable analysis

independent variables

CTAB method for genomic DNA extraction
(GTG)5 primer for repetitive sequence-based polymerase chain reaction (rep-PCR)

dependent variables

Amplicon fingerprint profiles analyzed using Gelcompar II software
Pearson correlation analysis of banding patterns
Dendrogram created using unweighted pair group method with arithmetic averages

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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