The immunofluorescence assay was performed according to the previously described methods [30 (link)]. The primary antibodies used were shown in Table 2. The secondary antibodies used were Alexa 488 goat-anti mouse lgG (1:1000, Invitrogen) and Alexa Fluor 594 donkey anti-rabbit IgG (1:1000, Invitrogen). The cells were analyzed under an LSM780 or LSM800 confocal microscope (Zeiss, Germany).
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