Immunofluorescence Staining Procedure

Primary antibodies: rabbit-anti-phospho-Smad1/5 (41D10, Cell Signaling, #9516; 1:200), mouse-anti-Wg (4D4, DSHB, University of Iowa, RRID:AB_528512; total staining: 1:120, extracellular staining: 1:120), mouse-anti-Ptc (DSHB, University of Iowa, RRID:AB_528441; total staining: 1:40), rat-anti-HA (3F10, Roche, 11867423001, RRID:AB_390914; total staining: 1:300, extracellular staining: 1:20), rat-anti-Ollas (L2, Novus Biologicals, NBP1-06713, RRID:AB_1968650; total staining: 1:300, extracellular staining: 1:20), mouse-anti-HS (F69-3G10, amsbio; 1:100).
The following secondary antibodies were used at 1:500 dilutions in this study. Goat anti-rabbit IgG (H+L) Alexa Fluor 488 (A11008 Thermo Fischer), goat anti-rabbit IgG (H+L) Alexa Fluor 568 (A11011 Thermo Fischer), goat anti-rabbit IgG (H+L) Alexa Fluor 680 (A21109 Thermo Fischer), goat anti-rat IgG (H+L) Alexa Fluor 568 (A11077 Thermo Fischer), goat anti-mouse IgG (H+L) Alexa Fluor 568 (A11004 Thermo Fischer), goat anti-rat IgG Fc 488 (ab97089 abcam), goat anti-mouse IgG Fc Alexa Fluor 680 (115625071 Jackson Immuno Research).

Free full text: Click here

Simon N., Safyan A., Pyrowolakis G, & Matsuda S. (2024). Dally is not essential for Dpp spreading or internalization but for Dpp stability by antagonizing Tkv-mediated Dpp internalization. eLife, 12, RP86663.

Publication 2024

Alexa Fluor 488 Alexa Fluor 568 Alexa Fluor 680

Corresponding Organization :

Other organizations : University of Basel, Max Planck Institute of Immunobiology and Epigenetics, University of Freiburg

Top 5 similar protocols

Variable analysis

independent variables

Rabbit-anti-phospho-Smad1/5 (41D10, Cell Signaling, #9516; 1:200)
Mouse-anti-Wg (4D4, DSHB, University of Iowa, RRID:AB_528512; total staining: 1:120, extracellular staining: 1:120)
Mouse-anti-Ptc (DSHB, University of Iowa, RRID:AB_528441; total staining: 1:40)
Rat-anti-HA (3F10, Roche, 11867423001, RRID:AB_390914; total staining: 1:300, extracellular staining: 1:20)
Rat-anti-Ollas (L2, Novus Biologicals, NBP1-06713, RRID:AB_1968650; total staining: 1:300, extracellular staining: 1:20)
Mouse-anti-HS (F69-3G10, amsbio; 1:100)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!