Imaging YFP-Gal8 in MDA-MB-231 Cells
Corresponding Organization :
Other organizations : Vanderbilt University, Vanderbilt University Medical Center
Variable analysis
- Treatment with media only
- Treatment with lipofectamine RNAiMax with 50 nM "zipper" siRNA against luciferase
- Treatment with 1 μM of si_Luc < (EG_18L)_2 in Opti-MEM
- YFP-Gal8 expression in MDA-MB-231 cells
- Cells were plated in a black 96-well plate 24 h prior to treatment
- NucBlue live cell nuclear stain (Invitrogen) was used
- Positive control: Cells treated with lipofectamine RNAiMax with 50 nM "zipper" siRNA against luciferase
- Negative control: Cells treated with media only
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!