MDA-MB-231 cells expressing YFP-Gal8 were generated as previously published68 (link) and plated in a black 96-well plate 24 h prior to treatment with media only, lipofectamine RNAiMax with 50 nM “zipper” siRNA against luciferase, or 1 μM of siLuc < (EG18L)2 in Opti-MEM with NucBlue live cell nuclear stain (Invitrogen). Cells were imaged at 18 h after initiation of treatment using a Nikon Ti confocal microscope.
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