Jejunum Digesta Viscosity Measurement

Following the procedure by Passos et al. (2015) (link) and Duarte et al. (2019) (link), samples of jejunum digesta from 50 mL tubes were divided into 2 falcon tubes (15 mL) and centrifuged at 1,000 × g at 4 °C for 10 min to obtain the liquid phase. The liquid phase was then removed and transferred to an Eppendorf tube (2 mL) to centrifuge at 10,000 × g at 4 °C for 10 min. The supernatant obtained was transferred to another Eppendorf tube (1.5 mL) for further measurement. About 0.5 mL of digesta supernatant was placed in the viscometer (Brookfield Digital Viscometer, Model DV-II Version 2.0, Brookfield Engineering Laboratories Inc., Stoughton, MA), set at 25 °C. The viscosity measurement was the average between 45.0 s⁻¹ and 22.5 s⁻¹ shear rates, and the viscosity values were recorded as apparent viscosity in centipoise (cP).