Measuring HUVEC Monolayer Integrity by TEER

HUVECs monolayer integrity was analyzed by TEER. Resistance was measured across the monolayer as described previously [20 (link)]. Briefly, 0.4 μM transwell inserts (Corning CoStar cooperation) were coated with collagen. HUVECs were plated at a density of 1 × 10⁵ cells/well. TEER was measured as described previously [21 (link),22 (link)]. Monolayers were treated with DMSO (control) for 12 h, TNF-α for 12 h, RVX208/JQ1 for 12 h, and pretreatment with RVX208/JQ1 for 12 h followed by TNF-α for 12 h. TEER was measured after 12 h treatments using the epithelial Volt/Ohm meter (EVOM2). Values were normalized against the resistance of a blank well containing only medium and coated with collagen.