Recombinant Bim1 and GST‐Bim1 and respective truncations were purified from E. coli according to the protocol described in Zimniak et al (2009 (link)) with the following modifications: Cleared cell lysates were loaded on a GSTrap column (GE Healthcare). Elution fractions were collected and subjected to buffer exchange using a HiPrep 26/30 Desalting column. The following purification steps were performed as described in previous publications.
Other organizations :
University of Duisburg-Essen, Max Planck Institute of Molecular Physiology, University of Münster, Ludwig-Maximilians-Universität München
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