Cultivation of BHK-21 Suspension Cells for FMDV

BHK-21 suspension cells were developed from the original adherent cell line, BHK-21 (C-13) (ATCC, Manassas, VA, USA) by the Animal and Plant Quarantine Agency and the Korea Research Institute of Bioscience & Biotechnology for use in suspension culture with serum-free media [15 ]. ProVero™ 1 cell culture medium (Lonza, Basel, Switzerland), a serum-free cell culture medium, was used to culture the BHK-21 suspension cells by incubation at 110 rpm in a shaking incubator at 37 °C with 5% CO₂. In this study, three different strains of serotype O FMDV were used. Two strains were domestic isolates called FMDV O/Boeun/SKR/2017 and FMDV O Jincheon/SKR/2014. The other strain was a genetically engineered virus called the FMDV Om-O-PanAsia2 recombinant strain that was made from a recombination of the backbone region of the O1 Manisa/Turkey/69 strain with the P1 region only being replaced by the O PAK/44/2008 strain [16 (link)].