Gelation behavior in the presence of drugs was tested by vial inverting assay, where no flow in the presence of gravitation implies formation of gel. For DTX, the required amount of gelator and DTX (from ethanol stock) was taken in a vial. The solvent was removed under nitrogen and dried under vacuum. The mixture was dissolved in water with heating and sonication and allowed to cool for gelation. CPT entrapment was studied simply by mixing the water-soluble CPT formulation with hydrogelator in water. Drug concentration below which A13 gelator retains its ability to form gel is considered as the maximum drug loading efficiency. Hydrogel loaded with combination of DTX and CPT was prepared by mixing DTX with A13 gelator followed by addition of aqueous solution of CPT. Resultant suspension was heated until the solution turned clear and was allowed to cool at room temperature. The drug-loaded heated solution was taken in a syringe, allowed to cool to form the gel, and was used for animal models using 22- to 26-gauze needle. Gels were characterized for rheology using a Rheoplus MCR102 (Anton-Paar, Graz, Austria) rheometer and for morphology using an ultra plus scanning electron microscope (Carl Zeiss, Germany) as per the published protocols (24 (link)).