Isolation and Characterization of Placental MSCs

Human placental tissues were obtained from healthy delivering women in accordance with the Austrian Hospital Act (KAG 1982) after written informed consent and the study was approved by the Ethic Commission of Lower Austria (GSl-EK-4/3122015). Amnion-derived MSCs from placental tissue were isolated and characterized with CD73-APC, CD90-FITC and CD105-PE-Cy7 (all from eBioscience, San Diego, CA) by flow cytometry (CytoFLEX XL, Beckman Coulter GmbH, Krefeld, Germany) as described previously [21 (link)] and HUVECs were isolated from the umbilical vein and characterized as published previously [24 (link)].

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Summer S., Rossmanith E., Pasztorek M., Fiedler C., Gröger M., Rauscher S., Weber V, & Fischer M.B. (2022). Mesenchymal stem cells support human vascular endothelial cells to form vascular sprouts in human platelet lysate-based matrices. PLOS ONE, 17(12), e0278895.

Publication 2022

CD73-APC CD90-FITC CD105-PE-Cy7 CytoFLEX XL

Amnion Cd73 Cd90 Fitc Flow cytometry Human Placental Tissue Umbilical vein Women

Corresponding Organization : Medical University of Vienna

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Variable analysis

independent variables

Amnion-derived MSCs from placental tissue
HUVECs isolated from the umbilical vein

dependent variables

Expression of CD73, CD90, and CD105 on amnion-derived MSCs
Characterization of HUVECs

control variables

Healthy delivering women in accordance with the Austrian Hospital Act (KAG 1982)
Written informed consent
Study approved by the Ethic Commission of Lower Austria (GSl-EK-4/3122015)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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