CD34+ Cell Expansion with hBMSCs

Human CML K562 cells and Human bonemesenchymal stem cells (hBMSCs) were acquired from the American Type Culture Collection (ATCC). hBMSCs were maintained in DMEM/F12 including 10% fetal bovine serum. K562 CD34⁺ cells were isolated by using anti-CD34 magnetic beads. CD34⁺ cells were incubated with or without hBMSCs at 37 °C with 5% CO₂ in high GF-supplemented(stem cell factor 100 pg/mL, SCF + FL + TPO, 50 ng/ml) serum-free expansion medium(Stem Cell Technologies, Vancouver, BC, Canada). Baicalein and IM were purchased from Melonepharma (Dalian, China). Anti-human GM-CSF (α-rhGM-CSF) was acquired from PeproTech Inc.(USA).