For each treatment, 30 healthy and mature leaves were selected, washed, and dried. Following a 30-min incubation at 105 °C, the leaves were dried at 75 °C, crushed, and screened. The ground leaves were collected in a self-sealing bag and stored in a dryer. Leaf samples were boiled in H2SO4–HClO4 and HNO3–HClO4 solutions and then the nutrient element contents were determined using AutoAnalyzer 3 with XY-2 Sampler (SEAL Analytical, UK) and an inductively coupled plasma emission spectrometer (Prodigy Spec, Teledyne, USA).
On August 10, 10 randomly selected leaves (per treatment) were obtained from OY saplings to measure the catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) contents as previously described (Wang et al., 2018 (link)). CAT was determined by monitoring the decomposition of H2O2. SOD was assayed by monitoring the inhibition of the photochemical reduction of nitro blue tetrazolium. POD was determined by monitoring the oxidation reaction of guaiacol.
On August 10, 10 randomly selected leaves (per treatment) were obtained from OY saplings to measure the catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) contents as previously described (Wang et al., 2018 (link)). CAT was determined by monitoring the decomposition of H2O2. SOD was assayed by monitoring the inhibition of the photochemical reduction of nitro blue tetrazolium. POD was determined by monitoring the oxidation reaction of guaiacol.
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