Quantification of TGF-β1, TNF-α, and MDA in Samples

TGF-β1, TNF-α, and MDA levels of the conditioned medium or protein secretion of the samples were determined by ELISA assay using the mouse TGF-β1 (Elabscience, E-EL-M0051, China), TNF-α (Elabscience, E-EL-M0049, China), and MDA (Elabscience, E-EL-0060, China) ELISA kits following manufacturer’s protocols, then the absorbance were all read at 450 nm using microplate spectrophotometer (Multiskan GO, Thermo Fisher Scientific, USA) (24 25 26 (link)).

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Widowati W., Tjokropranoto R., Onggowidjaja P., Widya Kusuma H.S., Wijayanti C.R., Marthania M., Yati A, & Rizal R. (2023). Protective effect of yacon leaves extract (Smallanthus sonchifolius (Poepp.) H. Rob) through antifibrosis, anti-inflammatory, and antioxidant mechanisms toward diabetic nephropathy. Research in Pharmaceutical Sciences, 18(3), 336-345.

Publication 2023

E-EL-M0051 E-EL-M0049 E-EL-0060 Multiskan GO

Assay Elisa Mouse Protein Secretion Tgf β1 Tnf α

Corresponding Organization :

Other organizations : Maranatha Christian University, University of Indonesia

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Variable analysis

independent variables

TGF-β1
TNF-α

dependent variables

Levels of TGF-β1, TNF-α, and MDA in the conditioned medium or protein secretion of the samples

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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