Histological Analysis of Acetabular Labrum

Acetabular labral samples were sliced to 3 mm thick specimens and were preserved for 2 d in 10% buffered formalin. Further, the specimens were embedded in paraffin and cut into 4 µm thick sections.
Haematoxylin and eosin staining was performed for histological analysis, and acetabular labrum degeneration was evaluated using the Krenn score for fibrocartilage degeneration in the meniscus and labrum (grades 0–3; Table 2) [17 (link),18 (link),29 (link)] by three blinded assessors who were expert hip surgeons.

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Sato Y., Tetsunaga T., Yamada K., Kawamura Y., Yoshida A, & Ozaki T. (2023). Expression of Acetabular Labral Vascular Endothelial Growth Factor and Nerve Growth Factor Is Directly Associated with Hip Osteoarthritis Pain: Investigation by Immunohistochemical Staining. International Journal of Molecular Sciences, 24(3), 2926.

Publication 2023

Acetabular Embedded paraffin Eosin Fibrocartilage Formalin Haematoxylin Meniscus Surgeons

Corresponding Organization : Okayama University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Acetabular labrum degeneration evaluated using the Krenn score for fibrocartilage degeneration in the meniscus and labrum (grades 0-3)

control variables

Acetabular labral samples were sliced to 3 mm thick specimens
Acetabular labral samples were preserved for 2 d in 10% buffered formalin
Acetabular labral samples were embedded in paraffin and cut into 4 µm thick sections

controls

Haematoxylin and eosin staining was performed for histological analysis
Three blinded assessors who were expert hip surgeons evaluated the acetabular labrum degeneration

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