Comprehensive Antibody Usage Protocol

The following antibodies were used in this study: H3K27me3 (Millipore 07-449), H3K27ac (Abcam ab4729, Western blot), H3K27ac (Cell signaling, 8173BC, ChIP), H3K4me1 (homemade EDL), H4K12ac (Millipore, 07-595), P300 (Clone NM11, Active Motif 61401), BRD4 (Bethyl A301-985A50, ChIP), BRD4 (Abcam ab128874, Western blot), Cas9 (Millipore MAC133), H2A.Z (Abcam ab150402), mH2A1 (Abcam ab37264, ChIP), mH2A1 (Millipore 07-219, Western blot), mH2A2 (Homemade, Bernstein Lab^{23 (link)}), H3 (Abcam Ab1791), H4 (Abcam, ab177840), GFP (Roche 11814460001), Beta-Actin (Sigma, A5441), Flag (Sigma, F1804), Mouse IgG – DyLight 680 (Invitrogen SA5-10170), Rabbit IgG DyLight 800 (Invitrogen SA5-10044). The antibodies used in this study are listed in Supplementary Table 2.

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Mohammed Ismail W., Mazzone A., Ghiraldini F.G., Kaur J., Bains M., Munankarmy A., Bagwell M.S., Safgren S.L., Moore-Weiss J., Buciuc M., Shimp L., Leach K.A., Duarte L.F., Nagi C.S., Carcamo S., Chung C.Y., Hasson D., Dadgar N., Zhong J., Lee J.H., Couch F.J., Revzin A., Ordog T., Bernstein E, & Gaspar-Maia A. (2023). MacroH2A histone variants modulate enhancer activity to repress oncogenic programs and cellular reprogramming. Communications Biology, 6, 215.

Publication 2023

H3K27me3 H3K27ac H3K4me1 H4K12ac mH2A1 Beta-Actin Flag

A301 Antibodies Beta actin Brd4 Chip Clone Mouse P300 Rabbit Western blot

Corresponding Organization :

Other organizations : Mayo Clinic, Icahn School of Medicine at Mount Sinai, Baylor College of Medicine, Mayo Clinic in Florida

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Variable analysis

independent variables

H3K27me3 (Millipore 07-449)
H3K27ac (Abcam ab4729, Western blot)
H3K27ac (Cell signaling, 8173BC, ChIP)
H3K4me1 (homemade EDL)
H4K12ac (Millipore, 07-595)
P300 (Clone NM11, Active Motif 61401)
BRD4 (Bethyl A301-985A50, ChIP)
BRD4 (Abcam ab128874, Western blot)
Cas9 (Millipore MAC133)
H2A.Z (Abcam ab150402)
MH2A1 (Abcam ab37264, ChIP)
MH2A1 (Millipore 07-219, Western blot)
MH2A2 (Homemade, Bernstein Lab23)
H3 (Abcam Ab1791)
H4 (Abcam, ab177840)
GFP (Roche 11814460001)
Beta-Actin (Sigma, A5441)
Flag (Sigma, F1804)

dependent variables

Not explicitly mentioned

control variables

Mouse IgG – DyLight 680 (Invitrogen SA5-10170)
Rabbit IgG DyLight 800 (Invitrogen SA5-10044)

Annotations

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