To stimulate caspase-11 noncanonical inflammasome activation, LPS was electroporated into indicated cells using the Neon Transfection System (Thermo Fisher Scientific) according to the manufacturer’s protocol. Briefly, BMDMs were electroporated with LPS (1 to 3 μg) in buffer R (no. MPK10025, Thermo Fisher Scientific) under pulse voltage of 1400 V, pulse width of 10 ms, and pulse number 2 (5 (link)).
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Stimulate Caspase-11 Inflammasome Activation
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Corresponding Organization :
Other organizations : Guangzhou Medical University, Xiangya Hospital Central South University, Central South University, Army Medical University, Daping Hospital, Third Xiangya Hospital, The University of Texas Southwestern Medical Center, Délégation Paris 5, Sorbonne Paris Cité, Université Paris Cité, Centre de Recherche des Cordeliers, Inserm, Sorbonne Université, Institut Gustave Roussy, Feinstein Institute for Medical Research, North Shore University Hospital, University of Pittsburgh
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Variable analysis
independent variables
- LPS concentration (1 to 3 μg)
- Caspase-11 noncanonical inflammasome activation
- Pulse voltage (1400 V)
- Pulse width (10 ms)
- Pulse number (2)
- No positive or negative controls were explicitly mentioned in the provided information.
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