Images of cochlear whole mounts and organotypic cultures were acquired using either a Yokogawa CSU-W1 spinning disk on a Nikon Ti2 microscope with a Hamamatsu Flash 4.0 V3 camera operated by NIS-Elements or a Nikon A1 confocal microscope, using ×100 or ×60 lenses. Exposure times were set to ensure high signal-to-noise ratio and no saturation in the image. Gain and offset adjusting were performed to ensure that no saturated or undersaturated pixels were present. Identical capture and analysis conditions were used for each experimental and control tissue.
Images were processed and three-dimensional renderings were generated using NIS-Elements and Imaris. Nuclear volumes were measured on the basis of DAPI fluorescence using Imaris, as detailed in ref. 2 (link).
Images were processed and three-dimensional renderings were generated using NIS-Elements and Imaris. Nuclear volumes were measured on the basis of DAPI fluorescence using Imaris, as detailed in ref. 2 (link).
