Evaluating HDAC Activity in Breast Tumors

Nuclear proteins from mouse breast tumor tissues were extracted by NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific). The HDACs activity was evaluated by EpiQuik HDAC Activity/Inhibition Assay Kit (Epigentek, Brooklyn, NY) according to the manufacturer’s protocols, respectively, as done previously (13 (link), 18 (link)). The enzymatic activities of HDACs were colorimetrically demonstrated and detected by an Epoch Microplate Spectrophotometer at 450 nm.

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Li Y., Buckhaults P., Li S, & Tollefsbol T.O. (2018). Temporal efficacy of a sulforaphane-based broccoli sprout diet in prevention of breast cancer through modulation of epigenetic mechanisms. Cancer prevention research (Philadelphia, Pa.), 11(8), 451-464.

Publication 2018

NE-PER Nuclear and Cytoplasmic Extraction Reagents EpiQuik HDAC Activity/Inhibition Assay Kit

Assay Breast tumor Cytoplasmic Enzymatic activities Epoch Inhibition Mouse Nuclear proteins Tissues

Corresponding Organization : University of Alabama at Birmingham

Other organizations : University of South Carolina

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Variable analysis

independent variables

Nuclear proteins from mouse breast tumor tissues

dependent variables

HDACs activity

control variables

Nuclear proteins extraction by NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific)
HDACs activity evaluation by EpiQuik HDAC Activity/Inhibition Assay Kit (Epigentek, Brooklyn, NY)
Enzymatic activities of HDACs detected by an Epoch Microplate Spectrophotometer at 450 nm

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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