An individualized IGHV germline gene database from macaque 25257 PBMCs was produced from total mRNA (53 ). IgDiscover was used as an inference tool (54 (link)) and libraries for high-throughput sequencing were produced by 5’ multiplex PCR as previously described (55 (link)). Briefly, mRNA was reverse transcribed with IgM constant region-specific primer containing a unique molecular identifier and a universal outer primer sequence. The cDNA was amplified utilizing the universal 3’ primer and two leader/UTR primer sets covering all gene families. Illumina indices and adapters were introduced by PCR prior to sequencing the library with Illumina’s MiSeq v3 kit. The output library was analyzed with IgDiscover (v0.10a) to infer the germline gene IGHV alleles (54 (link)). The initial database utilized for the analysis was obtained from genomic DNA sequencing (56 (link)) with the addition of non-located (NL) alleles inferred from a larger set of macaques (53 ). The heavy chain sequences of the 58 isolated mAbs were assigned to the IGHV individualized database from macaque 25257 and to the IMGT’s light chain database using IgBlast for analysis of SHM and CDR3 sequences. IGHJ sequences were identified using the IMGT rhesus macaque database.